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Impact of DNA Sequence and Oligonucleotide Length on a Polythiophene‐Based Fluorescent DNA Biosensor
Author(s) -
Charlebois Isabelle,
Gravel Catherine,
Arrad Naoual,
Boissinot Maurice,
Bergeron Michel G.,
Leclerc Mario
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200469
Subject(s) - oligonucleotide , dna , biosensor , sequence (biology) , biomolecule , polythiophene , oligomer restriction , biophysics , dna sequencing , chemistry , hybridization probe , repeated sequence , a dna , fluorescence , dna–dna hybridization , biochemistry , biology , polymer , genome , gene , optics , physics , organic chemistry , conductive polymer
DNA hybridization is a universal and specific mechanism for the recognition of biological targets. Some cationic polythiophene transducers sensitive to DNA structure have been previously utilized to detect such biomolecules. Further characterization of these systems indicates that both DNA sequence composition and length modulate the biosensor performance. It appears that different repeated sequence patterns cause different conformational changes of the polythiophene, from a more relaxed form to an extremely rigid one. A length difference between the DNA oligonucleotide probe and target has a detrimental effect on the fluorescent signal, but it can be attenuated by changing the sequence composition of the protruding target sequence. This demonstrates that the nature of DNA can be critical for hybridization‐based detection systems.