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Ni(II)‐NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical Properties and First In Vitro Study of Polyplexes Formed with HIV‐Derived Peptides
Author(s) -
Hauptmann Nicole,
Pion Marjorie,
MuñozFernández MaríaÁngeles,
Komber Hartmut,
Werner Carsten,
Voit Brigitte,
Appelhans Dietmar
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200449
Subject(s) - chemistry , in vitro , imine , peptide , biocompatible material , imidazole , polymer chemistry , combinatorial chemistry , biophysics , nuclear chemistry , organic chemistry , biochemistry , medicine , biomedical engineering , biology , catalysis
Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)‐nitrilo(triacetic acid)‐modified poly(ethylene imine)‐maltose ( Ni‐NTA‐DG ) is realized and evaluated as complexation agent against His‐tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5–6.0, and also up to 50 m M of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte‐derived immature dendritic cells. In summary, Ni‐NTA‐DG represents a promising (delivery) platform for forthcoming in vitro applications.