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Mastoid Obliteration Using Three‐Dimensional Composite Scaffolds Consisting of Polycaprolactone/β‐Tricalcium Phosphate/Collagen Nanofibers: An In Vitro and In Vivo Study
Author(s) -
Jang Chul Ho,
Cho Yong Beom,
Yeo Myung Gu,
Kim Geun Hyung
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200393
Subject(s) - scaffold , polycaprolactone , nanofiber , in vivo , biomedical engineering , regeneration (biology) , chemistry , composite number , materials science , composite material , microbiology and biotechnology , medicine , polymer , biology
Two different composite scaffolds, solid‐freeform‐fabricated PCL/β‐TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β‐TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular activities of osteoblast‐like‐cells (MG63), SEM images and MTT assays are conducted. Experimental mastoid obliteration is performed using guinea pigs that are divided group A (PCL/β‐TCP/collagen‐nanofiber scaffold) and group B (PCL/β‐TCP scaffold). The results reveal that PCL/β‐TCP/collagen scaffold provide much broader cell attachment sites than PCL/β‐TCP scaffold. The µ‐CT and fluorescent microscopy results reveal that the acceleration of early new bone formation within the pores and scaffold itself at week 4 post‐operation is more effective in group A. In addition, based on the results of the histological and µ‐CT at 12 weeks post‐surgery, the effective regeneration of bone in the PCL/β‐TCP/collagen scaffold is appeared.