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Direct Adhesion of Endothelial Cells to Bioinspired Poly(dopamine) Coating Through Endogenous Fibronectin and Integrin α 5 β 1
Author(s) -
Wang JinLei,
Ren KeFeng,
Chang Hao,
Jia Fan,
Li BoChao,
Ji Ying,
Ji Jian
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200390
Subject(s) - adhesion , fibronectin , umbilical vein , chemistry , coating , cell adhesion , endothelial stem cell , integrin , polycaprolactone , microbiology and biotechnology , biophysics , cytoskeleton , cell , nanotechnology , materials science , in vitro , biochemistry , biology , polymer , organic chemistry
Mussel‐inspired poly(dopamine) (PDA) coating is proven to be a simple, versatile, and effective strategy to promote cell adhesion onto various substrates. In this study, the initial adhesive behavior of human umbilical vein endothelial cells (HUVECs) is evaluated on a PDA coating under serum‐free conditions. It is found that HUVECs can attach directly to and spread with well‐organized cytoskeleton and fibrillar adhesions on the PDA surface, whereas cells adhere poorly to and barely spread on the control polycaprolactone surface. Endogenous fibronectin and α 5 β 1 integrin are found to be involved in the cell adhesion process. These findings will lead to a better understanding of interactions between cells and PDA coating, paving the way for the further development of PDA.