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Polymers for the Cell‐Specific Immobilisation of Megakaryocytic Cell Lines
Author(s) -
Hansen Anne,
Corless Samuel,
Cleland Alexander,
Petrik Juraj,
Gilbert Nick,
Bradley Mark
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200368
Subject(s) - flow cytometry , transplantation , megakaryocyte , cell , microbiology and biotechnology , chemistry , platelet , stem cell , precursor cell , cell therapy , biology , haematopoiesis , immunology , biochemistry , medicine , surgery
Primary human megakaryocytes, the precursor cells of platelets, are difficult to collect and cultivate. Polymers that enrich these cells without affecting their regulation or function are of interest for basic research as well as for cord blood transplantation purposes since co‐transplantation of enriched megakaryocyte concentrates increase the success of stem cell therapy. Herein, polymer microarrays were used for the discovery of substrates for MEG‐01 cells, with five polymers identified which selectively bound cells of the megakaryocytic lineage. Flow cytometry and miRNA profiling revealed that immobilisation had only a minor effect on the cellular maturation status, making the identified substrates potential candidates for concentrating megakaryocytes from patients prior to transplantation.