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Preparation of Functional Water‐Soluble Low‐Cytotoxic Poly(methacrylate)s With Pendant Cationic L ‐Lysines for Efficient Gene Delivery
Author(s) -
Sun Jingjing,
Luo Ting,
Sheng Ruilong,
Li Hui,
Chen Shengdian,
Hu Fangzhen,
Cao Amin
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200304
Subject(s) - transfection , hela , gene delivery , cationic polymerization , chemistry , intracellular , methacrylate , zeta potential , cytotoxicity , biophysics , microbiology and biotechnology , plasmid , biochemistry , dna , gene , biology , polymer chemistry , monomer , in vitro , materials science , polymer , organic chemistry , nanotechnology , nanoparticle
In this work, we present the preparation of water‐soluble poly(methacrylate)s with pendant cationic L ‐lysines PHMLs(6–30 K). Plasmid DNA binding affinity as well as particle sizes and zeta potentials of the polyplexes were examined for these PHML vectors, and their cytotoxicities were assayed with HeLa cells by CCK‐8 and lactate dehydrogenase kits. Gene transfection efficacy and intracellular uptake of the polyplexes by the PHML vectors were also studied with HeLa cells. As a result, it was revealed that the low cytotoxic PHMLs tended to exhibit gene transfection efficiencies significantly higher than those of the linear structural PLL (15–30 K) control, in particular the molecular weight of a PHML vector remarkably influenced its pDNA binding affinity, transfection efficacy and intracellular uptake of the polyplexes.