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In Vitro Transfection Mediated by Dendrigraft Poly( L ‐lysines): The Effect of Structure and Molecule Size
Author(s) -
Hofman Jakub,
Buncek Martin,
Haluza Radovan,
Streinz Ludvik,
Ledvina Miroslav,
Cigler Petr
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200303
Subject(s) - transfection , lipofectamine , oligonucleotide , in vitro , chemistry , gene delivery , plasmid , microbiology and biotechnology , dna , genetic enhancement , cell culture , biophysics , gene , vector (molecular biology) , recombinant dna , biology , biochemistry , genetics
Dendritic poly( L ‐lysines) (DGL) constitute promising nanomaterials applicable as a nonviral gene‐delivery vector. In this study, we evaluate the transfection abilities of four DGL generations with special emphasis on the systematic description of the relationship of how generation (i.e., molecule size) affects the transfection efficacy. Using Hep2 cells, we demonstrated that the capability of unmodified DGL to deliver plasmid is of a magnitude lower than that of jetPEI. On the other hand, employing the Hep2 cell line stably transduced with eGFP, we observed that DGL G5 delivers the siRNA oligonucleotide with the same efficiency as Lipofectamine 2000. In further experiments, it was shown that DGL affords excellent ability to bind DNA, protect it against DNase I attack, and internalize it into cells.