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Self‐Assembled BolA‐like Amphiphilic Peptides as Viral‐Mimetic Gene Vectors for Cancer Cell Targeted Gene Delivery
Author(s) -
Chen JingXiao,
Xu XiaoDing,
Yang Shuo,
Yang Juan,
Zhuo RenXi,
Zhang XianZheng
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200283
Subject(s) - amphiphile , gene delivery , capsid , moiety , hela , genetic enhancement , peptide , chemistry , gene , plasmid , microbiology and biotechnology , cell , biochemistry , biology , stereochemistry , copolymer , organic chemistry , polymer
In this study, two types of BolA‐like amphiphilic peptides with dual ligands comprising a tumor‐targeting moiety of RGD sequence and a cell‐penetrating moiety of R8 sequence are designed and synthesized as gene vectors. The BolA‐structural peptide carriers can self‐assemble into spherical nanoparticles with a hydrophilic core and shell, which are similar to the viral capsid and can bind plasmid DNA in an aqueous medium to form viral‐mimetic complexes. It is found that the BolA‐like dual ligands system exhibits significantly enhanced gene expression in both HeLa and 293T cell lines, as compared with poly(ethylenimine) PEI. These BolA‐like amphiphilic peptides are promising in clinical trials of gene therapy.