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Polymer Carriers for Anticancer Drugs Targeted to EGF Receptor
Author(s) -
Studenovsky Martin,
Pola Robert,
Pechar Michal,
Etrych Tomas,
Ulbrich Karel,
Kovar Lubomir,
Kabesova Martina,
Rihova Blanka
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200270
Subject(s) - methacrylamide , conjugate , oligopeptide , chemistry , biophysics , receptor , ligand (biochemistry) , cancer research , polymer , copolymer , biochemistry , peptide , biology , acrylamide , mathematics , organic chemistry , mathematical analysis
Abstract A novel actively targeted polymer carrier for anticancer drugs based on an N ‐(2‐hydroxypropyl)methacrylamide copolymer (PHPMA) is proposed. An oligopeptide sequence GE7, attached to the polymer, is a specific ligand for the EGF receptor overexpressed on most tumor cells. Co‐attachment of selected chemotherapeutics will therefore lead to formation of tumor‐specific polymer therapeutics, further enhanced by the EPR effect. FACS measurements prove elevated binding activity of the fluorescently labeled PHPMA/GE7 conjugate in EGFR‐rich cells (FaDu, MCF‐7), compared to conjugates of scrambled peptides. Cell lines with low EGFR level (SW620, B16F10) bind the GE7 conjugate significantly less.