Premium
In vitro 3D Full‐Thickness Skin‐Equivalent Tissue Model Using Silk and Collagen Biomaterials
Author(s) -
Bellas Evangelia,
Seiberg Miri,
Garlick Jonathan,
Kaplan David L.
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200262
Subject(s) - dermis , skin equivalent , epidermis (zoology) , silk , in vitro , keratin , adipose tissue , chemistry , biomedical engineering , anatomy , microbiology and biotechnology , materials science , pathology , keratinocyte , biology , biochemistry , medicine , composite material
Current approaches to skin equivalents often only include the epidermis and dermis. Here, a full‐thickness skin equivalent is described including epidermis, dermis, and hypodermis, that could serve as an in vitro model for studying skin biology or as a platform for consumer product testing. The construct is easy to handle and is maintained for >14 d while expressing physiological morphologies of the epidermis and dermis, seen by keratin 10, collagens I and IV expression. The skin equivalent produces glycerol and leptin, markers of adipose metabolism. This work serves as a foundation for understanding a few necessary factors needed to develop a stable, functional model of full‐thickness skin.