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A Serum‐Resistant Low‐Generation Polyamidoamine with PEI 423 Outer Layer for Gene Delivery Vector
Author(s) -
Pan Shirong,
Cao Duanwen,
Huang Huan,
Yi Wu,
Qin Linhao,
Feng Min
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200255
Subject(s) - polyethylenimine , transfection , gene delivery , chemistry , zeta potential , cytotoxicity , nuclease , polymer chemistry , biophysics , polymer , nuclear chemistry , microbiology and biotechnology , nanotechnology , nanoparticle , dna , materials science , gene , biochemistry , organic chemistry , in vitro , biology
A new derivative of polyamidoamine and polyethylenimine, G2.5‐PEI 423 or G1.5‐PEI 423, is prepared by an amidation reaction of PAMAM G2.5 or PAMAM G1.5 using PEI 423. The polycations show a great ability to combine with pDNA to form complexes, which protect the pDNA from nuclease degradation. The polymers display stronger buffer capacity and lower cytotoxicity. The complexes have particle sizes of 120–180 nm and zeta potentials of 20–40 mV. The G2.5‐PEI 423 complexes display much higher transfection efficiencies than PAMAM G5 and Lipo‐2k, and the G1.5‐PEI 423 complexes display higher transfection efficiencies than PAMAM G4 and PEI‐25k. The complexes possess better serum‐resistant capacity. The G2.5‐PEI 423 has a great potential to be used as a serum‐resistant gene vector.