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Study of Intracellular Delivery of Doxorubicin from Poly(lactide‐ co ‐glycolide) Nanoparticles by Means of Fluorescence Lifetime Imaging and Confocal Raman Microscopy
Author(s) -
Romero Gabriela,
Qiu Yuan,
Murray Richard A.,
Moya Sergio E.
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200235
Subject(s) - plga , confocal microscopy , fluorescence microscope , flow cytometry , confocal , chemistry , biophysics , doxorubicin , fluorescence lifetime imaging microscopy , viability assay , nanoparticle , fluorescence , bovine serum albumin , intracellular , fluorescence correlation spectroscopy , materials science , nanotechnology , cell , microbiology and biotechnology , chromatography , biochemistry , medicine , biology , physics , surgery , organic chemistry , chemotherapy , molecule , mathematics , geometry , quantum mechanics
The intracellular delivery of Doxorubicin (Dox) from poly(lactide‐ co ‐glycolide) (PLGA) nanoparticles stabilised with bovine serum albumin, in HepG2 cells, is studied via flow cytometry, fluorescence lifetime imaging microscopy (FLIM), confocal Raman microscopy (CRM) and cell viability studies. Flow cytometry shows that the initial uptake of PLGA and Dox follow the same kinetics. However, following 8 h of incubation, the fluorescence intensity and cellular uptake of Dox decreases, while in the case of PLGA both parameters remain constant. FLIM shows the presence of a single‐lifetime species, with a lifetime of 1.15 ns when measured inside the cells. Cell viability decreases by approximately 20% when incubated for 24 h with PLGA loaded with Dox, with a particle concentration of 100 µg · mL −1 . At the single‐cell level, CRM shows changes in the bands from DNA and proteins in the cell nucleus when incubated with PLGA loaded with Dox.