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Mass‐producible Nano‐featured Polystyrene Surfaces for Regulating the Differentiation of Human Adipose‐derived Stem Cells
Author(s) -
Park KwangSook,
Cha Kyoung Je,
Han InBo,
Shin DongAh,
Cho DongWoo,
Lee SooHong,
Kim Dong Sung
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200225
Subject(s) - nanopillar , substrate (aquarium) , stem cell , adipogenesis , chemistry , adhesion , polystyrene , microbiology and biotechnology , adipose tissue , focal adhesion , regenerative medicine , biophysics , nanotechnology , materials science , biochemistry , nanostructure , cell , biology , polymer , ecology , organic chemistry
In this study, we report an efficient and cost‐effective method of fabricating polystyrene (PS) nano‐featured substrates containing nanopore (NPo) and nanopillar (NPi) arrays based on hot embossing using nickel nano‐stamps. We investigate the behavior of adipose‐derived stem cells (ASCs), including adhesion, morphology, proliferation and differentiation, on the replicated PS surfaces. Compared to a flat substrate, NPo‐ and NPi‐featured substrates do not alter the morphology of stem cells. However, both NPo‐ and NPi‐featured substrates induce different integrin expression and lower formation of focal adhesion complexes. In addition, ASCs on the NPo‐featured substrate exhibit greater adipogenic differentiation, while the NPi‐featured substrate induces higher osteogenic differentiation.