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Functionalized Polystyrene Nanoparticles Trigger Human Dendritic Cell Maturation Resulting in Enhanced CD4 + T Cell Activation
Author(s) -
Frick Stefanie U.,
Bacher Nicole,
Baier Grit,
Mailänder Volker,
Landfester Katharina,
Steinbrink Kerstin
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200223
Subject(s) - dendritic cell , chemistry , downregulation and upregulation , t cell , monocyte , cell , polystyrene , immunotherapy , microbiology and biotechnology , cell growth , biophysics , immune system , immunology , biochemistry , biology , polymer , organic chemistry , gene
Nanoparticles (NP) represent a promising tool for biomedical applications. Here, sulfonate‐ and phosphonate‐functionalized polystyrene NP are analyzed for their interaction with human monocyte‐derived dendritic cells (DC). Immature dendritic cells (iDC) display a higher time‐ and dose‐dependent uptake of functionalized polystyrene NP compared to mature dendritic cells (mDC). Notably, NP induce an enhanced maturation of iDC but not of mDC (upregulation of stimulatory molecules and cytokines). NP‐triggered maturation results in a significantly enhanced T cell stimulatory capacity (increased CD4 + T cell proliferation and IFN‐γ production), indicating a shift to a pronounced Th1 response. Immunomodulatory properties of NP may be a useful strategy for strengthening the efficacy of NP‐based approaches in immunotherapy.