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Impact of Peptide Micropatterning on Endothelial Cell Actin Remodeling for Cell Alignment under Shear Stress
Author(s) -
Chollet Céline,
Bareille Reine,
Rémy Murielle,
Guignandon Alain,
Bordenave Laurence,
Laroche Gaetan,
Durrieu MarieChristine
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200167
Subject(s) - micropatterning , shear stress , biophysics , adhesion , cell adhesion , homogeneous , cell , materials science , chemistry , actin , shear (geology) , focal adhesion , endothelial stem cell , peptide , in vitro , nanotechnology , composite material , biochemistry , biology , physics , thermodynamics
Abstract HSVEC behavior under physiological shear stress in vitro is investigated on PET surfaces micropatterned with both RGDS and WQPPRARI peptides. This technique allows (i) creating geometries on surface to guide cell orientation under shear stress and (ii) controlling surface chemical composition in order to modulate cell behavior. Under shear stress, endothelial cells adhere on patterned PET surfaces and present a more rapid orientation in flow direction in comparison to cells cultured on homogeneous surfaces. Micropatterned surfaces presenting a large surface area ratio of RGDS/WQPPRARI peptides induce fibrillar adhesion, while surfaces presenting an equal RGDS/WQPPRARI peptides surface area ratio preferentially induce focal adhesion.