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Preparation of Biomimetic Hydrogels with Controlled Cell Adhesive Properties and Topographical Features for the Study of Muscle Cell Adhesion and Proliferation
Author(s) -
Jun Indong,
Kim Seok Joo,
Choi Eunpyo,
Park Kyung Min,
Rhim Taiyoun,
Park Jungyul,
Park Ki Dong,
Shin Heungsoo
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200148
Subject(s) - self healing hydrogels , c2c12 , adhesion , cell adhesion , biophysics , myocyte , chemistry , cell , adhesive , cell growth , substrate (aquarium) , peptide , tissue engineering , morphology (biology) , materials science , nanotechnology , microbiology and biotechnology , polymer chemistry , biochemistry , biomedical engineering , biology , organic chemistry , myogenesis , medicine , ecology , genetics , layer (electronics)
Synthetic substrates with defined chemical and structural characteristics may potentially be prepared to mimic the living ECM to regulate cell adhesion and growth. Hydrogels with cell‐adhesive peptides (0.28 ± 0.03 nmol peptide · cm −2 , TTA‐R‐0.5; and 0.91 ± 0.12 nmol peptide · cm −2 , TTA‐R‐2.0) and/or micro‐scaled topographical patterns (10, 25, and 80 µm grooves) are prepared using enzymatic polymerization. The adherent morphology and proliferation of C2C12 skeletal myoblasts and human aortic smooth muscle cells (hAoSM) on the hydrogels are studied. The newly developed hydrogels may be useful in investigating the roles of cell adhesion and substrate surface properties in the communication of adherent cells with the ECM.