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“Click & Seed” Approach to the Biomimetic Modification of Material Surfaces
Author(s) -
Proks Vladimír,
Jaroš Josef,
PopGeorgievski Ognen,
Kučka Jan,
Popelka Štěpán,
Dvořák Petr,
Hampl Aleš,
Rypáček František
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200095
Subject(s) - azide , surface modification , peptide , cycloaddition , substrate (aquarium) , chemistry , combinatorial chemistry , click chemistry , alkyne , chemical modification , polymer brush , polymer chemistry , biophysics , nanotechnology , catalysis , materials science , organic chemistry , biochemistry , polymerization , polymer , biology , oceanography , geology
Abstract A simple, versatile, protein‐repulsive, substrate‐independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow‐up modifications with various ligands using a copper‐catalyzed alkyne‐azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125 I‐radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line.