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Modified Hyaluronan Hydrogels Support the Maintenance of Mouse Embryonic Stem Cells and Human Induced Pluripotent Stem Cells
Author(s) -
Liu Yongxing,
Charles Lyndon F.,
Zarembinski Thomas I.,
Johnson Kalle I.,
Atzet Sarah K.,
Wesselschmidt Robin L.,
Wight Mark E.,
Kuhn Liisa T.
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200043
Subject(s) - induced pluripotent stem cell , embryonic stem cell , self healing hydrogels , microbiology and biotechnology , stem cell , matrigel , kosr , chemistry , gelatin , flow cytometry , biology , cell , biochemistry , gene , organic chemistry
These studies provide evidence for the ability of a commercially available, defined, hyaluronan‐gelatin hydrogel, HyStem‐C™, to maintain both mouse embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs) in culture while retaining their growth and pluripotent characteristics. Growth curve and doubling time analysis show that mESCs and hiPSCs grow at similar rates on HyStem‐C™ hydrogels and mouse embryonic fibroblasts and Matrigel™, respectively. Immunocytochemistry, flow cytometry, gene expression and karyotyping reveal that both human and murine pluripotent cells retain a high level of pluripotency on the hydrogels after multiple passages. The addition of fibronectin to HyStem‐C™ enabled the attachment of hiPSCs in a xeno‐free, fully defined medium.