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Fluorescence‐Labeled Immunomicelles: Preparation, in vivo Biodistribution, and Ability to Cross the Blood–Brain Barrier
Author(s) -
Yue Jun,
Liu Shi,
Wang Rui,
Hu Xiuli,
Xie Zhigang,
Huang Yubin,
Jing Xiabin
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200037
Subject(s) - micelle , biodistribution , chemistry , blood–brain barrier , conjugated system , in vivo , amphiphile , biophysics , drug carrier , drug delivery , in vitro , copolymer , biochemistry , organic chemistry , biology , polymer , microbiology and biotechnology , neuroscience , aqueous solution , central nervous system
Abstract Multifunctional hybrid micelles are prepared from amphiphilic mal‐PEG ‐b‐ PLA and mPEG ‐b‐ P(LA ‐co‐ DHC/RhB) block copolymers. A specific anti‐transferrin receptor antibody, OX26, is linked onto the surface of the micelles. ELISA indicates that the conjugated antibody preserves its activity. OX26 conjugation can increase the uptake efficiency of micelles by target cell lines (C6). Pharmacokinetics and in vivo biodistribution experiments are carried out to investigate the ability of OX26‐conjugated micelles (immunomicelles) to cross the blood–brain barrier. The data show that the brain uptake of OX26‐conjugated micelles is much more than that of OX26‐free ones. Therefore, OX26‐conjugated micelles will be promising drug carriers to cross the blood‐brain barrier.