Premium
Design of Serum Compatible Tetrary Complexes for Gene Delivery
Author(s) -
Bahadur K. C. Remant,
Thapa Bindu,
Xu Peisheng
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100464
Subject(s) - transfection , cationic polymerization , chemistry , gene delivery , ternary operation , biophysics , ternary complex , adsorption , gene , biochemistry , polymer chemistry , organic chemistry , biology , enzyme , computer science , programming language
A novel gene delivery system, called PoSC, consisting of PEI, PSP, and HA is described. In contrast to the DNA/PEI/HA ternary system whose transfection efficiency decreases significantly with increasing serum concentration, PoSC exhibits a high transfection efficiency of about 51 and 87% for NIH3T3 and HCT116 cells, respectively, at 50% serum concentration. Furthermore, PoSC shows no cytotoxic effect at its working concentration. The overall results suggest that HA adsorption on cationic complexes enhances the transfection efficiency, while PSP is essential for high transfection efficiency at higher serum concentration.