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Signaling Pathways of Immobilized FGF‐2 on Silicon‐Substituted Hydroxyapatite
Author(s) -
de la Concepción Matesanz María,
Feito María José,
RamírezSantillán Cecilia,
Lozano Rosa María,
SánchezSalcedo Sandra,
Arcos Daniel,
ValletRegí María,
Portolés MaríaTeresa
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100456
Subject(s) - fibroblast growth factor , biomaterial , osteoblast , chemistry , mapk/erk pathway , fibroblast , microbiology and biotechnology , signal transduction , intracellular , cell growth , basic fibroblast growth factor , regeneration (biology) , cell , biophysics , growth factor , biochemistry , in vitro , biology , receptor , organic chemistry
Therapeutic strategies for bone regeneration involve the selection of suitable biomaterials, growth factors, and cell types to mimic the cellular microenvironment where molecular and mechanical signals control the reconstruction of bone tissue. The immobilization of basic fibroblast growth factor (FGF‐2) on powdered silicon‐substituted hydroxyapatite (Si‐HA) allows to prepare a biofunctional biomaterial able to interact with bone cells in a very specific way. The biological activity of FGF‐2/Si‐HA, evaluated in Saos‐2 osteoblasts and MC3T3‐E1 preosteoblasts through the PLCγ and MAPK/ERK signal transduction pathways, shows that FGF‐2 immobilized on Si‐HA provides the right signals to cells stimulating crucial intracellular mechanisms of osteoblast proliferation and differentiation.