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Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression
Author(s) -
Lammers Gerwen,
Roth Günter,
Heck Mathias,
Zengerle Roland,
Tjabringa G. Sandra,
Versteeg Elly M.,
Hafmans Theo,
Wismans Ronnie,
Reinhardt Dieter P.,
Verwiel Eugene T. P.,
Zeeuwen Patrick L. J. M.,
Schalkwijk Joost,
Brock Roland,
Daamen Willeke F.,
van Kuppevelt Toin H.
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100443
Subject(s) - papillary dermis , dermis , morphology (biology) , keratinocyte , chemistry , pathology , microbiology and biotechnology , biophysics , biology , medicine , biochemistry , in vitro , genetics
A papillary‐structured collagen fibril membrane is created, mimicking the 3D‐architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar‐structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT‐qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.