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PEGylated Click Polypeptides Synthesized by Copper‐Free Microwave‐Assisted Thermal Click Polymerization for Selective Endotoxin Removal from Protein Solutions
Author(s) -
Guo Jinshan,
Meng Fanbo,
Li Xiaoyuan,
Wang Mingzhe,
Wu Yanjuan,
Jing Xiabin,
Huang Yubin
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100394
Subject(s) - click chemistry , copper , polymerization , chemistry , combinatorial chemistry , polymer chemistry , chemical engineering , organic chemistry , polymer , engineering
PEGylated click polypeptides ( PEG‐CP s) containing α ‐amino side groups as well as PEG segments are designed for selective endotoxin removal from protein solutions. The PEG‐CP s are synthesized via copper‐free thermal click copolymerization from aspartic (or glutamic) acid‐based dialkyne and diazide monomers (containing free amino side groups) and alkyne‐terminated mPEGs or dialkyne‐terminated PEGs. Microwave‐assisting technology is introduced into thermal click chemistry to improve the reaction efficiency. The monomers and polymers are fully characterized using NMR, XPS, and MALDI‐TOF MS. After immobilizing the PEGylated click polypeptides onto polystyrene microspheres, the adsorbents exhibit good endotoxin removal selectivity from BSA solutions.