Premium
New Monodisperse Magnetic Polymer Microspheres Biofunctionalized for Enzyme Catalysis and Bioaffinity Separations
Author(s) -
Horák Daniel,
Kučerová Jana,
Korecká Lucie,
Jankovičová Barbora,
Palarčík Jiří,
Mikulášek Petr,
Bílková Zuzana
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100393
Subject(s) - dispersity , chemistry , immobilized enzyme , polymer , covalent bond , chemical engineering , polymerization , microsphere , magnetic nanoparticles , polymer chemistry , zeta potential , nanotechnology , organic chemistry , enzyme , materials science , nanoparticle , engineering
Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi‐step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta‐potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin‐modified magnetic PGMA‐COOH and PHEMA‐COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.