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Encapsulation and Survival of a Chondrocyte Cell Line within Xanthan Gum Derivative
Author(s) -
Mendes Ana C.,
Baran Erkan T.,
Pereira Rui C.,
Azevedo Helena S.,
Reis Rui L.
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100304
Subject(s) - xanthan gum , cell encapsulation , chondrocyte , polysaccharide , chemistry , chondrogenesis , cell culture , homogeneous , biophysics , chemical engineering , cell , materials science , biochemistry , composite material , in vitro , biology , genetics , physics , rheology , engineering , thermodynamics
A chemical derivative of xanthan gum polysaccharide is investigated as a new artificial matrix for the encapsulation of chondrocytic cells. Toward this goal, a novel micro‐droplet generator is developed to produce microcapsules. Microcapsules with an average diameter of 500 µm, smooth surface, and homogeneous size distribution are obtained. ATDC5 cells encapsulated in carboxymethyl xanthan (CMX) microcapsules remain viable and are observed to proliferate for prolonged culture periods with enhanced metabolic activity. Furthermore, retention of the chondrogenic phenotype is exhibited by the cells within CMX, suggesting the ability of this material to be applied in cell‐delivery therapies.