Premium
Thermally Modulated Retention of Lymphoctytes on Polymer‐Brush‐Grafted Glass Beads
Author(s) -
Nagase Kenichi,
Mukae Naoto,
Kikuchi Akihiko,
Okano Teruo
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100283
Subject(s) - brush , wetting , polymer brush , bead , polymer chemistry , contact angle , materials science , chemical engineering , polymer , chemistry , composite material , polymerization , engineering
Thermoresponsive PIPAAm‐brush‐grafted glass beads are prepared through siATRP and their physicochemical properties are characterized by micro‐nitrogen analysis, XPS, and contact angle measurements. The amount of grafted PIPAAm on glass bead surfaces can be controlled by varying the ATRP reaction time, leading to a modulation of the temperature‐dependent wettability of the prepared surfaces. To evaluate a possible use of the beads as cell separation matrices, loading with rat lymphocytes from mesenteric lymph nodes is studied. The results show that the interaction between PIPAAm brushes and lymphocytes can be controlled by modulating PIPAAm brush length and temperature. The PIPAAm‐brush‐grafted beads might therefore be useful as effective cell separation matrices.