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Silk‐Based Nanocomplexes with Tumor‐Homing Peptides for Tumor‐Specific Gene Delivery
Author(s) -
Numata Keiji,
MieszawskaCzajkowska Aneta J.,
Kvenvold Laura A.,
Kaplan David L.
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100274
Subject(s) - transfection , gene delivery , chemistry , cytotoxicity , in vitro , microbiology and biotechnology , silk , cytotoxic t cell , melanoma , recombinant dna , lysine , biochemistry , gene , cancer research , biology , amino acid , materials science , composite material
Nanoscale complexes of recombinant silk molecules containing THPs with DNA are designed as less cytotoxic and highly target‐specific gene carriers. Genetically engineered silk proteins containing poly( L ‐lysine) domains to interact with pDNA and the THP to bind to specific tumorigenic cells for target‐specific pDNA delivery are prepared, followed by in vitro transfection into MDA‐MB‐435 melanoma cells, highly metastatic human breast tumor MDA‐MB‐231 cells, and non‐tumorigenic MCF‐10A breast epithelial cells. The silk/poly( L ‐lysine) block copolymer containing Lyp1 (ML‐Lyp1) shows significant differences from silk/poly( L ‐lysine) block copolymer containing F3 (ML‐F3) in cytotoxicity to MCF10A cells. ML‐F3 is the most promising candidate for target delivery into tumorigenic cells.