Premium
Polymeric Fibrous Matrices for Substrate‐Mediated Human Embryonic Stem Cell Lineage Differentiation
Author(s) -
Cooper Ashleigh,
Leung Matthew,
Zhang Miqin
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100269
Subject(s) - embryonic stem cell , microbiology and biotechnology , cellular differentiation , induced pluripotent stem cell , chemistry , substrate (aquarium) , anatomy , biophysics , nanotechnology , materials science , biology , biochemistry , ecology , gene
Chitosan‐based fibrous matrices are prepared to mimic the ECM architecture and elucidate substrate‐mediated hESC differentiation due to topographical scale and anisotropy without exogenic morphogens. Fibrous matrices support fewer pluripotent hESCs than films but enable topography‐mediated hESC differentiation. Matrices composed of 400 nm and 1.1 µm diameter fibers support increased expression of neural markers indicative of ectodermal commitment while matrices of 200 nm diameter fibers increase expression of osteogenic and hepatic markers indicative of endodermal and mesodermal commitment. The fibrous‐mediated hESC differentiation highlights the significant implication of tailored ECM‐like substrates for hESC‐based therapies.