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Controlled Release of DNA From Photoresponsive Hyperbranched Polyglycerols with Oligoamine Shells
Author(s) -
Fischer Wiebke,
Quadir Mohiuddin A.,
Barnard Anna,
Smith David K.,
Haag Rainer
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100248
Subject(s) - ethidium bromide , methylamine , chemistry , surface modification , polymer , nanoparticle , polymer chemistry , bromide , dynamic light scattering , chemical engineering , dna , photochemistry , materials science , nanotechnology , organic chemistry , biochemistry , engineering
Two photo‐responsive core/shell nanoparticles based on hyperbranched polyglycerol (hPG) are synthesized for controlled release of DNA. The shell is composed either of bis‐(3‐aminopropyl)methylamine (AMPA) or pentaethylenehexamine (PEHA) derivatives and is attached to the hPG core with a photo‐responsive o ‐nitrobenzyl linker. Ethidium bromide displacement assay, gel electrophoresis, DLS, and ζ‐potential measurements are performed with these nanoparticles. Photo‐responsive changes within the carrier scaffold are investigated by irradiating the polymer solution with 350 nm monochromatic light. Fully covered APMA‐shelled carriers are found to complex the DNA at an N/P ratio of 10 with an average size ranging from 54 to 78 nm depending on the degree of functionalization of the core.