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Bilayer Matrix Composed of Polycation/DNA Complex and Sodium Alginate Gel as a Tumor Cell Catcher
Author(s) -
Zeng Xuan,
Sun YunXia,
Qu Wei,
Zhuo RenXi,
Zhang XianZheng
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100212
Subject(s) - transfection , bilayer , hela , chemistry , dna , cell , matrix (chemical analysis) , cell culture , cell adhesion , adhesion , biophysics , microbiology and biotechnology , biochemistry , biology , membrane , gene , genetics , chromatography , organic chemistry
A bilayer matrix consisting of TABP‐SS/DNA complexes and sodium alginate gel is formed via electrostatic interaction. In vitro cell adhesion, proliferation and transfection of the bilayer matrix are investigated in HepG2, HeLa and COS7 cells. Results show that this matrix can only promote tumor cell attachment and growth. Compared with normal cells, the bilayer matrix exhibits significantly higher transfection efficacy in tumor cells. Cell co‐culture competitive transfection assay shows that the cell uptake of TABP‐SS/DNA complexes is significantly enhanced in tumor cells rather than normal cells under the co‐culture competitive condition, which confirms that TABP‐SS/DNA complexes have strong tumor cell selectivity and tumor targeting transfection ability.