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Tissue‐Specific Stem Cell Differentiation in an in vitro Airway Model
Author(s) -
Prytherch Zoë,
Job Claire,
Marshall Hilary,
Oreffo Victor,
Foster Martyn,
BéruBé Kelly
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100181
Subject(s) - respiratory epithelium , epithelium , respiratory tract , serous fluid , microbiology and biotechnology , in vitro , progenitor cell , stem cell , basal (medicine) , cellular differentiation , pathology , biology , cell culture , goblet cell , immunology , respiratory system , anatomy , medicine , endocrinology , biochemistry , genetics , gene , insulin
The respiratory tract is the primary site of exposure to airborne compounds, with the bronchial epithelium providing one of the first lines of defence. A growing need exists for an accurate in vitro model of the bronchial epithelium. Here, normal human bronchial epithelial (NHBE) cells cultured at an air/liquid interface create a fully differentiated, in‐vivo‐like model of the human bronchial epithelium. Developmental characterisation includes (i) trans‐ epithelial electrical resistance, (ii) morphology and (iii) bronchial cell specific stains/markers. It is concluded that the basal/progenitor cells create a pseudo‐stratified, mucociliary NHBE model containing basal, serous, Clara, goblet and ciliated cells, reflective of the normal human bronchial epithelium (days 24–33 ALI culture).