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Core/Shell Fe 3 O 4 @SiO 2 Nanoparticles Modified with PAH as a Vector for EGFP Plasmid DNA Delivery into HeLa Cells
Author(s) -
Shi Mengran,
Liu Yiyao,
Xu Mingming,
Yang Hong,
Wu Chunhui,
Miyoshi Hirokazu
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100150
Subject(s) - hela , transfection , plasmid , nanoparticle , dna , chemistry , gene delivery , microbiology and biotechnology , biophysics , cytotoxicity , green fluorescent protein , viability assay , oligonucleotide , cell , nanotechnology , materials science , biochemistry , in vitro , gene , biology
Novel stable core/shell Fe 3 O 4 @SiO 2 /PAH nanoparticles are synthesized using 15 nm Fe 3 O 4 as the template that is modified with PAH. The resulting nanoparticles can absorb plasmid DNA to mediate gene transfer in cultured HeLa cells. An electrophoretic assay suggests that the Fe 3 O 4 @SiO 2 /PAH nanoparticles protect the plasmid DNA from serum and DNase I degradation. A cell viability assay shows that the Fe 3 O 4 @SiO 2 /PAH nanoparticles exhibit a low cytotoxicity toward endothelial cells. Qualitative analysis of transfection in HeLa cells by nanoparticles carrying a plasmid DNA encoding EGFP demonstrates a fairly high expression level, even in the presence of serum. Thus, Fe 3 O 4 @SiO 2 /PAH nanoparticles are biocompatible and suitable for nonviral delivery, and may find applications in cancer therapy.