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Bio‐electrosprayed Living Composite Matrix Implanted into Mouse Models
Author(s) -
Jayasinghe Suwan N.,
Warnes Gary,
Scotton Chris J.
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100131
Subject(s) - in vivo , in vitro , composite number , biophysics , electrospinning , biopolymer , chemistry , matrix (chemical analysis) , microbiology and biotechnology , cell , biomedical engineering , nanotechnology , materials science , biology , biochemistry , polymer , composite material , medicine , chromatography , organic chemistry
We show that composite de novo structures can be generated using bio‐electrosprays. Mouse lung fibroblasts are bio‐electrosprayed directly with a biopolymer to form cell‐bearing matrices, which are viable even when implanted subcutaneously into murine hosts. Generated cell‐bearing matrices are assessed in‐vitro and found to undergo all expected cellular behaviour. Subsequent in‐vivo studies demonstrate the implanted living matrices integrating as expected with the surrounding microenvironment. The in‐vitro and in‐vivo studies elucidate and validate the ability for either bio‐electrosprays or cell electrospinning to form a desired living architecture for undergoing investigation for repairing, replacing and rejuvenating damaged and/or ageing tissues.