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Enhancement of Muramyldipeptide (MDP) Immunostimulatory Activity by Controlled Multimerization on Dendrimers
Author(s) -
Sorensen Nanna S.,
Boas Ulrik,
Heegaard Peter M. H.
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100105
Subject(s) - dendrimer , peptidoglycan , chemistry , monomer , innate immune system , mhc class i , immune system , peripheral blood mononuclear cell , microbiology and biotechnology , biophysics , biochemistry , major histocompatibility complex , immunology , in vitro , biology , enzyme , polymer , gene , receptor , organic chemistry
Peptidoglycan is a widespread bacterial PAMP molecule and a powerful initiator of innate immune responses. It consists of repeating units of MDP, which as a monomer is only weakly immunostimulatory. Here, MDP‐coupled dendrimers were prepared and investigated for stimulation of pig blood mononuclear cells. Compared to monomeric MDP, MDP‐dendrimers induced a markedly enhanced production of IL‐12 p40, IL‐1β and IL‐6 and completely down‐regulated surface expression of B7 and MHC class II. These results suggest a possible novel strategy based on controlled multimerization of minimal PAMP motifs on dendrimers for preparing molecularly defined immunostimulators with predictable bioactivities.