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Thermoresponsive Gene Carriers Based on Polyethylenimine ‐graft‐ Poly[oligo(ethylene glycol) methacrylate]
Author(s) -
Zhang Rui,
Wang Yang,
Du FuSheng,
Wang YingLi,
Tan YingXia,
Ji ShouPing,
Li ZiChen
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100094
Subject(s) - polyethylenimine , cationic polymerization , ethylene glycol , methacrylate , polymer chemistry , chemistry , copolymer , cytotoxicity , polyelectrolyte , transfection , lower critical solution temperature , zeta potential , gene delivery , biophysics , polymer , materials science , organic chemistry , nanotechnology , biochemistry , in vitro , nanoparticle , gene , biology
A family of thermoresponsive cationic copolymers (TCPs) that contain branched PEI 25 K as the cationic segment and poly(MEO 2 MA ‐co‐ OEGMA 475 ) as the thermosensitive block (TP) is prepared. The DNA binding capability, physicochemical properties, and biological performance of the TCPs are studied. All of these TCPs can condense DNA to form polyplexes with diameters of 150–300 nm and zeta potentials of 7–32 mV at N/P ratios between 12 and 36. The length of TP block is a key factor for shielding the positive surface charge of the polyplexes and protecting them against protein adsorption. TCPs with a higher TP content have a lower cytotoxicity while the best transfection performance is achieved by the TCPs with longest TP length, reaching a level of the intact PEI 25 K in the presence of serum.