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Topography‐Induced Cell Adhesion to Acr‐sP(EO ‐stat‐ PO) Hydrogels: The Role of Protein Adsorption
Author(s) -
Schulte Vera A.,
Diez Mar,
Möller Martin,
Lensen Marga C.
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100087
Subject(s) - self healing hydrogels , adsorption , chemistry , protein adsorption , adhesion , stat , cell adhesion , biophysics , polymer chemistry , chemical engineering , biochemistry , phosphorylation , organic chemistry , stat3 , biology , engineering
Abstract Topographic surface patterning of intrinsically non‐adhesive P(EO ‐stat‐ PO)‐based hydrogels can lead to the adhesion and spreading of fibroblasts. Explanations for this unexpected behavior are discussed, particularly with regard to non‐specific protein adsorption from the serum‐supplemented culture medium. The presence of serum proteins is shown to be essential for adhesion. Adsorption of plasma and ECM proteins (Fibronectin (FN) and Vitronectin (VN)) to the hydrogels is possible. The effect of VN on initial cell adhesion is analyzed in detail. It appears that VN is the main serum component that is crucial for initial cell adhesion to PEG and that surface topography is essential for further, durable adhesion establishment, and spreading.