Premium
Patterned Hydrogel Substrates for Cell Culture with Electrohydrodynamic Jet Printing
Author(s) -
Poellmann Michael J.,
Barton Kira L.,
Mishra Sandipan,
Johnson Amy J. Wagoner
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201100004
Subject(s) - mechanobiology , self healing hydrogels , microcontact printing , nanotechnology , materials science , electrohydrodynamics , substrate (aquarium) , polyacrylamide , biophysics , chemistry , microbiology and biotechnology , biology , polymer chemistry , ecology , electrode
Cells respond to and are directed by physiochemical cues in their microenvironment, including geometry and substrate stiffness. The development of substrates for cell culture with precisely controlled physiochemical characteristics has the potential to advance the understanding of cell biology considerably. In this communication, E‐jet printing is introduced as a method for creating high‐resolution protein patterns on substrates with controlled elasticity. It is the first application of E‐jet printing on a soft surface. Protein spots as small as 5 µm in diameter on polyacrylamide are demonstrated. The patterned hydrogels are shown to support cell attachment and spreading. Polyacrylamide substrates patterned by E‐jet printing may be applied to further the study of cellular mechanobiology.