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Neuron Cells Uptake of Polymeric Microcapsules and Subsequent Intracellular Release
Author(s) -
Pavlov Anton M.,
Sapelkin Andrei V.,
Huang Xinyue,
P'ng Ken M. Y.,
Bushby Andy J.,
Sukhorukov Gleb B.,
Skirtach André G.
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000494
Subject(s) - intracellular , biophysics , neuron , cytosol , chemistry , in situ , nanotechnology , microscopy , materials science , biochemistry , neuroscience , biology , organic chemistry , optics , enzyme , physics
Neuron cells uptake of biodegradable and synthetic polymeric microcapsules functionalized with aggregates of gold nanoparticles incorporated into their shells is demonstrated in situ. In addition to traditionally used optical microscopy, electron microscopy is used both for higher‐resolution imaging and for confirming the uptake by focused ion beam cross‐sectioning of specific cells in situ. Subsequently, physical methods of release are compared to chemical methods wherein laser‐induced intracellular release of dextran molecules into the cytosol of hippocampal neuron cells is studied in comparison to biodegradation. Implications of this work for neuroscience, bio‐medicine and single cell studies are discussed.