Premium
Cytokine Loaded Biopolymers as a Novel Strategy to Study Stem Cells during Wound‐Healing Processes
Author(s) -
Grimaldi Annalisa,
Banfi Serena,
Vizioli Jacopo,
Tettamanti Gianluca,
Noonan Douglas M.,
de Eguileor Magda
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000452
Subject(s) - matrigel , wound healing , microbiology and biotechnology , cytokine , stem cell , myofibroblast , biopolymer , biology , cell , in vivo , chemistry , immunology , biochemistry , pathology , fibrosis , medicine , polymer , organic chemistry
Abstract The biopolymer matrigel loaded with cytokine can be used for the recruitment in vivo of specific cell populations and as a vector for the preparation of cell cultures. Data demonstrate that the injection of the matrigel biopolymer supplemented with interleukin‐8 (IL‐8) in the leech Hirudo medicinalis can be used to purify cell populations showing the same morphofunctional and molecular mechanisms of specific populations of vertebrate hematopoietic precursor cells involved in tissue repair. These cells spontaneously differentiated into myofibroblasts. This approach highlights how the innovative use of a cytokine‐loaded biopolymer for an in vivo cell sorting method, applied to a simple invertebrate model, can be a tool for studying myofibroblast cell biology and its regulation, step by step.