Premium
Extracellular Matrix Functionalized Microcavities to Control Hematopoietic Stem and Progenitor Cell Fate
Author(s) -
Kurth Ina,
Franke Katja,
Pompe Tilo,
Bornhäuser Martin,
Werner Carsten
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000432
Subject(s) - extracellular matrix , microbiology and biotechnology , progenitor cell , haematopoiesis , stem cell , cell fate determination , progenitor , chemistry , matrix (chemical analysis) , in vitro , cell adhesion , cell , biology , biochemistry , chromatography , transcription factor , gene
Polymeric microcavities functionalized with extracellular matrix components were used as an experimental in vitro model to investigate principles of hematopoietic stem and progenitor cell (HSPC) fate control. Using human CD133+ HSPC we could demonstrate distinct differences in HSPC cycling and differentiation dependence on the adhesion ligand specificity (i.e., heparin, collagen I) and cytokine levels. The presented microcavity platform provides a powerful in vitro approach to explore the role of exogenous cues in HSPC fate decisions and can therefore be instrumental to progress in stem cell biology and translational research toward new therapies.