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Synthesis of Poly(propylene glycol)‐ block ‐Polyethylenimine Triblock Copolymers for the Delivery of Nucleic Acids
Author(s) -
Brissault Blandine,
Leborgne Christian,
Scherman Daniel,
Guis Christine,
Kichler Antoine
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000404
Subject(s) - transfection , polyethylenimine , nucleic acid , amphiphile , chemistry , gene delivery , small interfering rna , copolymer , dna , biophysics , ethylene glycol , polymer , microbiology and biotechnology , biochemistry , organic chemistry , gene , biology
LPEIs, which are efficient DNA transfection agents, were found to be far less effective for the delivery of siRNAs. Here, two amphiphilic triblock copolymers LPEI 50 ‐ b ‐PPG 36 ‐ b ‐LPEI 50 ( 2 ) and LPEI 14 ‐ b ‐PPG 68 ‐ b ‐LPEI 14 ( 4 ) have been synthesized. The transfection assays showed that compound 2 was efficient for DNA transfection whilst it was almost inactive for siRNA delivery. In contrast, polymer 4 was inefficient for DNA transfection while it showed capabilities for siRNA delivery. Taken together, our results indicate that the properties required for DNA and siRNA delivery are different. Moreover, we show that introduction of a hydrophobic segment that allows self‐assembly confers siRNA delivery capacities.