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BSA Adsorption on Differently Charged Polystyrene Nanoparticles using Isothermal Titration Calorimetry and the Influence on Cellular Uptake
Author(s) -
Baier Grit,
Costa Cristiane,
Zeller Anke,
Baumann Daniela,
Sayer Claudia,
Araujo Pedro H. H.,
Mailänder Volker,
Musyanovych Anna,
Landfester Katharina
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000395
Subject(s) - isothermal titration calorimetry , adsorption , surface charge , nanoparticle , chemistry , zeta potential , enthalpy , particle size , polystyrene , particle (ecology) , titration , chemical engineering , thermodynamics , organic chemistry , polymer , physics , oceanography , engineering , geology
BSA adsorption onto negatively and positively charged polystyrene nanoparticles was investigated. The nanoparticles were characterized in terms of particle size, zeta potential, surface group density, and morphology. The adsorption behavior of BSA on the particle surface, as a function of pH and overall charge of the particle, was studied using ITC. Different thermodynamic data such as enthalpy changes upon binding and stoichiometry of the systems were determined and discussed. The degree of surface coverage with BSA was calculated using the thermodynamic data. The cellular uptake of particles before and after BSA adsorption was studied using HeLa cells in the presence and absence of supplemented FCS in the cell culture medium.