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Preparation of 3D Fibroin/Chitosan Blend Porous Scaffold for Tissue Engineering Via a Simplified Method
Author(s) -
Ruan Yuhui,
Lin Hong,
Yao Jinrong,
Chen Zhengrong,
Shao Zhengzhong
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000392
Subject(s) - fibroin , scaffold , chitosan , tissue engineering , porosity , chemical engineering , materials science , reagent , chemistry , biomedical engineering , composite material , silk , organic chemistry , medicine , engineering
In this work, we developed a simple and flexible method to manufacture a 3D porous scaffold based on the blend of regenerated silk fibroin (RSF) and chitosan (CS). No crosslinker or other toxic reagents were used in this method. The pores of resulted 3D scaffolds were connected with each other, and their sizes could be easily controlled by the concentration of the mixed solution. Compared with pure RSF scaffolds, the water absorptivities of these RSF/CS blend scaffolds with significantly enhanced mechanical properties were greatly increased. The results of MTT and RT‐PCR tests indicated that the chondrocytes grew very well in these blend RSF/CS porous scaffolds. This suggested that the RSF/CS blend scaffold prepared by this new method could be a promising candidate for applications in tissue engineering.