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Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling‐Circle Amplification
Author(s) -
Barbee Kristopher D.,
Chandrangsu Matt,
Huang Xiaohua
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000373
Subject(s) - micropatterning , photolithography , polymer , photoresist , materials science , rolling circle replication , microfluidics , oligonucleotide , fabrication , nanotechnology , etching (microfabrication) , resist , lithography , layer (electronics) , chemistry , dna , composite material , optoelectronics , medicine , biochemistry , alternative medicine , pathology , dna replication
A method for fabricating DNA polymer brush arrays using photolithography and plasma etching followed by solid‐phase enzymatic DNA amplification is reported. After attaching oligonucleotide primers to the surface of a glass coverslip, a thin layer of photoresist is spin‐coated on the glass and patterned via photolithography to generate an array of posts in the resist. An oxygen‐based plasma is then used to destroy the exposed oligonucleotide primers. The glass coverslip with the primer array is assembled into a microfluidic chip and DNA polymer brushes are synthesized on the oligonucleotide array by rolling‐circle DNA amplification. We have demonstrated that the linear polymers can be rapidly synthesized in situ with a high degree of control over their density and length.