Premium
Hydrophobic Polyphenylalanine‐Grafted Hyperbranched Polyethylenimine and its in vitro Gene Transfection
Author(s) -
Xia Jialiang,
Chen Lei,
Chen Jie,
Tian Huayu,
Li Feifan,
Zhu Xiaojuan,
Li Gao,
Chen Xuesi
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000302
Subject(s) - polyethylenimine , transfection , hela , gene delivery , amphiphile , chemistry , copolymer , mtt assay , in vitro , zeta potential , cytotoxicity , microbiology and biotechnology , biophysics , polymer chemistry , biochemistry , polymer , organic chemistry , gene , nanotechnology , materials science , nanoparticle , biology
A series of amphiphilic multi‐armed PP n copolymers were prepared by ROP of Phe‐NCA with PEI‐25k as a macroinitiator. The particle size of the PP n /DNA complexes was about 100 nm and the zeta potentials were below 20 mV. An MTT assay demonstrated that all the PP n copolymers had lower cytotoxicity compared to PEI‐25k. In vitro gene transfection studies were also conducted in HeLa, 293 and CT 26 cells. The optimal quantity of hydrophobic phenylalanine segments in PP80 led to higher transfection efficiency in various cell lines based on this study. The results indicate that PP80 was the best candidate for gene delivery among these PP n copolymers.