Premium
Reversible Conformational Transitions of a Polymer Brush Containing Boronic Acid and its Interaction with Mucin Glycoprotein
Author(s) -
Ivanov Alexander E.,
Solodukhidezda,
Wahlgren Marie,
Nilsson Lars,
Vikhrov Alexander A.,
Nikitin Maxim P.,
Orlov Alexey V.,
Nikitin Petr I.,
Kuzimenkova Marina V.,
Zubov Vitali P.
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000295
Subject(s) - polyelectrolyte , polymer brush , chemistry , polymer , polymer chemistry , copolymer , phenylboronic acid , brush , ellipsometry , aqueous solution , chemical engineering , biophysics , materials science , organic chemistry , nanotechnology , thin film , engineering , composite material , polymerization , catalysis , biology
Abstract Reversible changes of the height of a polymer brush containing phenylboronic acid were studied. The polymer brush thickness underwent reversible changes of 0.5–1 nm, in response to the changes in composition of the contacting aqueous phase from deionized water to bicarbonate buffer and vice versa, apparently due to the conformational transition of the weak polyelectrolyte to the more extended electrically charged state. Adsorption of mucin glycoprotein to the polymer brush took place due to boronate/sugar interactions between the glycoprotein and the graft copolymer and resulted in further increase of the brush height by ca. 1.5 nm, as observed by means of spectral correlation spectroscopy and ellipsometry.