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Chemo‐Physical and Biological Evaluation of Poly( L ‐lysine)‐Grafted Chitosan Copolymers Used for Highly Efficient Gene Delivery
Author(s) -
Yu Haijun,
Deng Chao,
Tian Huayu,
Lu Tiancheng,
Chen Xuesi,
Jing Xiabin
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000283
Subject(s) - chitosan , copolymer , gene delivery , biocompatibility , transfection , chemistry , genetic enhancement , in vivo , gene transfer , in vitro , biophysics , polymer , materials science , polymer chemistry , gene , biochemistry , organic chemistry , biology , microbiology and biotechnology
For the success of non‐viral gene delivery, it is of great importance to develop gene vectors with high efficiency but low toxicity. We demonstrate that PLL‐grafted chitosan copolymers combine the advantages of PLL with its good pDNA‐binding ability and of chitosan with its good biocompatibility. The chemo‐physical properties of the prepared Chi ‐g‐ PLL copolymers are thoroughly characterized. The in vitro transfection study shows that the copolymers have a much higher gene transfer ability than the starting materials chitosan and PLL. A positive correlation between PLL chain lengths and transfection efficiency of the copolymers is found. Our results suggest that these novel Chi ‐g‐ PLL copolymers are good candidates for gene delivery in vivo.