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Modification of Polydivinylbenzene Microspheres by a Hydrobromination/Click‐Chemistry Protocol and their Protein‐Adsorption Properties
Author(s) -
Durmaz Yasemin Yuksel,
Karagoz Bunyamin,
Bicak Niyazi,
Demirkol Dilek Odaci,
Yalcinkaya Esra Evrim,
Timur Suna,
Yagci Yusuf
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000280
Subject(s) - adsorption , click chemistry , chemistry , protein adsorption , fourier transform infrared spectroscopy , desorption , polymer , microsphere , polymer chemistry , chemical engineering , organic chemistry , engineering
Hydrophobic‐ and/or hydrophilic‐polymer‐grafted PDVB microspheres are synthesized by the combination of hydrobromination and click‐chemistry processes. The modified‐PDVB microspheres and the intermediates at various stages of synthesis are characterized using GPC, 1 H NMR and FTIR spectroscopy and TGA analysis. Use of the microspheres as a support matrix for reversible protein immobilization via adsorption is investigated. The system parameters such as the adsorption conditions (i.e., enzyme concentration, medium pH) and desorption are studied and evaluated with regards to the biocatalytic activity and adsorption capacity.