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Amphiphilic Cationic [Dendritic poly( L ‐lysine)]‐ block ‐poly( L ‐lactide) ‐block‐ [dendritic poly( L ‐lysine)]s in Aqueous Solution: Self‐Aggregation and Interaction with DNA as Gene Delivery Carriers
Author(s) -
Zhu Yingdan,
Sheng Ruilong,
Luo Ting,
Li Hui,
Sun Wenyan,
Li Yang,
Cao Amin
Publication year - 2011
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000225
Subject(s) - amphiphile , cationic polymerization , chemistry , lysine , aqueous solution , transfection , polymer chemistry , hela , block (permutation group theory) , biophysics , polymer , copolymer , cell , biochemistry , organic chemistry , amino acid , gene , geometry , mathematics , biology
A new series of triblock [dendritic poly( L ‐lysine)]‐ block ‐PLLA‐ block ‐[dendritic poly( L ‐lysine)]s (DL 2 ‐PLLA‐DL 2 ) with PLLA block lengths of 11.5–26.5 and double 2‐generation PLL dendrons DL 2 as model cationic amphiphiles were synthesized and characterized. Their CAC, self‐aggregation and plasmid DNA binding affinities in pure water and PBS were studied. The PLLA block length dependence of particle size, morphology and ξ potential for organized pDNA/amphiphile polyplex aggregates were examined. Finally, toxicities of these DL 2 ‐PLLA‐DL 2 amphiphiles and their polyplexes were assayed by MTT with HeLa, SMMC‐7721 and COS‐7 cells, and COS‐7 cell luciferase and eGFP gene transfection efficacies with these amphiphiles as the delivery carriers were investigated.