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Inhibitory Effect of Hydrophilic Polymer Brushes on Surface‐Induced Platelet Activation and Adhesion
Author(s) -
Zou Yuquan,
Lai Benjamin F.L.,
Kizhakkedathu Jayachandran N.,
Brooks Donald E.
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000223
Subject(s) - polymer chemistry , atom transfer radical polymerization , dispersity , platelet activation , platelet , polymer brush , polymerization , chemistry , adhesion , polymer , vinyl chloride , chemical engineering , materials science , biophysics , organic chemistry , copolymer , immunology , engineering , biology
Poly( N , N ‐dimethylacrylamide) (PDMA) brushes are successfully grown from unplasticized poly(vinyl chloride) (uPVC) by well‐controlled surface‐initiated atom transfer radical polymerization (SI‐ATRP). Molecular weights of the grafted PDMA brushes vary from ≈ 35 000 to 2 170 000 Da, while the graft density ranges from 0.08 to 1.13 chains · nm −2 . The polydispersity of the grafted PDMA brushes is controlled within 1.20 to 1.80. Platelet activation (expression of CD62) and adhesion studies reveal that the graft densities of the PDMA brushes play an important role in controlling interfacial properties. PDMA brushes with graft densities between 0.35 and 0.50 chains · nm −2 induce a significantly reduced platelet activation compared to unmodified uPVC. Moreover, the surface adhesion of platelets on uPVC is significantly reduced by the densely grafted PDMA brushes. PDMA brushes that have high molecular weights lead to a relatively lower platelet activation compared to low‐molecular‐weight brushes. However, the graft density of the brush is more important than molecular weight in controlling platelet interactions with PVC. PDMA brushes do not produce any significant platelet consumption in platelet rich plasma. Up to a seven‐fold decrease in the number of platelets adhered on high graft density brushes is observed compared to the bare PVC surface. Unlike the bare PVC, platelets do not form pseudopodes or change morphology on PDMA brush‐coated surfaces.